Figure 2. Sequential Activation of
ref-2,
ttx-3,
ceh-10, and the AIY Motif. (A) Expression pattern of a
ref-2::venus rescuing translational fusion gene, observed with the transgenic array otEx3091. (Ai-Ai'') Expression in the SMDD/AIY mother (SMDD/AIYm) at the end of gastrulation (ventral view anterior is left, scale bar = 10 um, scheme adapted from Sulston et al. [1983]). (Aii) Expression of REF-2::VENUS in the SMDD/AIY lineage (marked by
ttx-3promB::mcherry, otIs181) before (interphase), during (mitosis), and after (postmitosis) cleavage of the SMDD/AIY mother (scale bar = 2 um). (Aiii and Aiii') Expression in the excretory pore cell (Exc pore) and excretory gland (Exc gld) at L2 larval stage (lateral view, anterior is left, dorsal is up, scale bar = 10 um). (Aiv) Expression in the P, Y, and B cells at L1 larval stage (lateral view, anterior is left, dorsal is up, scale bar = 20 um).(B) Comparison of the expression of
ttx-3 (
ttx-3promB::gfp, mgIs18 or
ttx-3promB::mcherry, otEx2644),
ceh-10 (
ceh-10promA::gfp, lqIs4), and the AIY motif (AIY motif::gfp, otEx1597) at the end of gastrulation (End gast., ventral view, anterior is left), 1.5-fold, and 3-fold (lateral view, anterior is left). At 1.5-fold, the
ttx-3 and
ceh-10 pictures show colocalization of
ttx-3promB::mcherry and
ceh-10promA::gfp expression in AIY in the same embryo (scale bar = 10 um).