Figure 2. Expression Analysis of
ceh-14. (A) Fluorescent photomicrographs of a transgenic adult hermaphrodite carrying pHK-108 and the
rol-6 marker, which causes a twisted body: the anterior to the left shows a dorsal-ventral view, the central region a more lateral view. The cells expressing CEH-14::GFP are the thermosensory neurons AFDL/R, the interneurons BDUL/R, and the asymmetric interneuron ALA. These cells were identified on their characteristic morphology and the position of their nuclei viewed by simultaneous fluorescence and Nomarski differential interference microscopy. Scale bar, 40 μm.(B) Schematic drawing of the cells expressing
ceh-14 in the head, lateral view.(C) Immunolocalization using anti-CEH-14 serum: the antibody detects AFD as well as the other neurons expressing CEH-14 (data not shown). Scale bar for (C) and (D), 12 μm.(D) Superposition of antibody immunolocalization and DAPI nuclear staining of the same animal. The antibody staining is nuclear, confirming that
ceh-14 is a transcription factor and localized as expected.