S7 Fig. Ciliary and socket cell structures in the
bgnt-1 mutant are present and appear superficially wild-type. (A) Both amphid and phasmid cilia appear superficially wild-type. GFPtagged CHE-2 (mammalian IFT80 orthologue) is used as a pan-cilia marker which localises to the basal bodies (bb) and axonemes. (B) ADL cilia correctly enter the amphid socket (AMso) cells in
bgnt-1.1 mutants. ADL cilia are labelled with Psrh-220::IFT-20::tdTomato. IFT-20(IFT20) localises to cilia basal bodies (bb) and axonemes. The
srh-220 promoter drives expression primarily in ADL neurons. The amphid socket (AMso) cells are labelled with cytoplasmic GFP driven by an amphid socket specific promoter,
grd-15 (Hunt-Newbury et al. 2007). (C) ADL cilia penetrate the sockets cells to an equivalent depth in wild-type and
bgnt-1.1 mutants (p > 0.05, Kruskal-Wallis test). (D) ADL cilia/dendrites exhibit no guidance defects in
bgnt-1.1 mutants as assessed by the number of double-rod cilia observed in each amphid when ADL was driven by the primarily ADL specific
srh-220 promoter (p > 0.05, Fisher's exact test). Error bars represent 95% confidence intervals (Pearson Clopper method). (E)
bgnt-1.1 mutants do not exhibit a significant increase in the proportion of ADL neurons with dendritic blebbing (p > 0.05, Fisher's exact test). Error bars represent 95% confidence intervals (Pearson Clopper method).