Figure 2.
ctn-1, an a-catulin homologue, has two distinct roles in mediating SLO-1 function. (A) The gene structure of
ctn-1. Our genomeanalysis indicates that unlike WormBase annotation (WS210)
ctn-1 consists of 13 exons and is predicted to encode a 784 amino-acid protein (FigureS1). The homology regions with a-catenin/vinculin I (homologous to the N-terminal talin/a-actinin-binding region of vinculin, 213 amino acids), acatenin/vinculin II (homologous to the C-terminal F-actin/inositol phospholipids-binding region of vinculin, 145 amino acids) and the coiled-coildomain (35 amino acids) are depicted at the bottom of the gene structure. The mutation sites for two different alleles (
eg1167 and
cim6) are shownon the top of the gene structure. The predicted amino acid sequence is available in Figure S1. (B) Rescue of the head bending phenotype with avariety of
ctn-1 constructs. Ex[
ctn-1] represents the transgene carrying the genomic
ctn-1 DNA extrachromosomal array. Ex[Pmyo-3ctn-1] represents themuscle-specific
myo-3 promoter-driven
ctn-1 transgene, whereas Ex[PH20ctn-1] represents the neuron-specific H20 promoter-driven
ctn-1 transgene.Error bars represent s. e. m. (n = 10). Single asterisks indicate significant difference between two groups (P,0.001, unpaired two-tailed t-test) whereasns indicates no significant difference. (C) Rescue of the defect in egg-laying muscle with a variety of
ctn-1 constructs. Error bars represent s. e. m.(n = 15). Single asterisks indicate significant difference between two groups (P,0.001, unpaired two-tailed t-test) whereas ns indicates no significantdifference. (D) Rescue of the average locomotory speed with a variety of
ctn-1 constructs. Error bars represent s. e. m. (n.10). Single asterisks indicatesignificant difference between two groups (P,0.001, unpaired two-tailed t-test) whereas ns indicates no significant difference. (E-H) The expressionpattern of a
ctn-1 promoter-tagged GFP reporter. Expression in (E) body wall muscles and the ventral cord neurons (arrowheads), (F) nerve ring(arrowheads) and pharyngeal muscle (arrow), (G) egg-laying muscle, and (H) enteric (arrow) and sphincter (arrowhead) muscle. Scale bar, 10 um.