Figure 7. SYP-1 phosphorylation is required for correct localization of CPC components (AIR-2 and ICP-1) and the CPC-guiding histone mark (H3T3ph).(A) Localization of GFP::AIR-2 (green) on meiotic chromosomes fixed and stained with DAPI (magenta) at different stages preceding and during the meiotic divisions in control (gfp::
air-2)(top) and gfp::
air-2;
syp-1(12A);
syp-1(
me17) (bottom) animals. Bars, 5 um. (B) ICP-1 is enriched on short arms in -1 oocytes in
syp-1(wt);
syp-1(
me17) animals (top), whereas some -1 oocytes lack ICP-1 localization in
syp-1(12A);
syp-1(
me17) mutants (bottom; see alsothe whole-nucleus image in Fig. S4). The ICP-1 localization in -1 oocytes was categorized as follows in
syp-1(wt);
syp-1(
me17),
syp-1(12A);
syp-1(
me17),and
plk-2(
ok1936): all six DAPI bodies had robust ICP-1 staining on short arms (category 1), some DAPI bodies had partial or weak ICP-1 staining (category 2), or no DAPI bodies had ICP-1 staining (category 3). The analysis was limited to -1 oocyte nuclei carrying six bivalents. Bars, 1 um. (C) H3T3phstaining in -1 oocyte nuclei in N2 wild-type (top) and
syp-1(12A);
syp-1(
me17) mutants (bottom); representative images are shown above quantitation(see also the whole-nucleus image in Fig. S4). Bars, 1 um. In the scatterplot below, each point is a measurement of a single DAPI body. Error bars indicatemean and SD of all points. The number of DAPI bodies/points counted for H3T3ph is 151 for wild type and 111 for
syp-1(12A) mutants. ****, P <0.0001, Mann-Whitney test.