FIGURE 6:
samp-1(RNAi) animals have a weak nuclear migration defect. (A-F) Embryos were stained for SAMP-1localization. Lateral views, with anterior left and dorsal up. Scale bars, 10 μm. For each pair of images, SAMP-1immunostaining is shown in white on the left and in red on the right when it is merged with DAPI staining of nuclei in blue. (A, B) An early wild-type embryo. (C, D) A later, pre-comma-stage embryo. Arrowhead points to a
hyp7 precursor nucleus. (E, F) A
samp-1(
tm2710)-null embryo is shown to demonstrate specificity of the antibody. (G) Numbers of nuclei in the dorsal cords of wild-type or
samp-1(
tm2710)/(+);
samp-1(RNAi) L1 larvae. Each gray dot represents an individual animal. The mean and 95% CI error bars are shown. (H, I) DIC and GFP images showing two
hyp7 nuclei abnormally in the dorsal cord (arrows) of a
samp-1(
tm2710)/(+);
samp-1(RNAi) L1 larva. The dorsal cord is up and is demarcated by the dotted line. Scale bar, 10 μm.