Figure 2. Immunostaining and
unc-68::gfp expression pattern in wild-type and
unc-68 mutant animals. Confocal images of each tissue in wild-type ((a), (d), (f), (h) and (k)),
unc-68(
x14) ((b) and (i)) and
him-8(
e1489) (m)) stained with the R16 antibody. Expression patterns of the
unc-68::gfp transgenic animal are also shown as confocal images ((c), (e), (g), (j) and (l)). Each panel shows the following: L4 worm ((a), (b) and (c)), head region ((h), (i) and (l)), body wall muscles ((d) and (e)), vulval muscles ((f) and (g)) and anal muscle of the hermaphrodite ((k) and (l)) and male tail ((m)). Arrows in (a) and (b) indicate the staining in intestine. Note that there is no difference between wild-type and
x14 animals. The R16 antibody stained the isthmus and terminal bulb in pharynx of wild-type ((h), arrowheads), but not
x14 animals ((d), arrowheads). The labeling pattern in the muscles of body wall ((d) and (e)) and vulva ((f) and (g)) was seen to be composed of a series of small dots. Note that the signal was seen in the depressor muscle ((h), open arrowheads) but not in the sphincter muscle of the defecation muscles ((h), arrowhead). Bar represents 50 um ((d), Bar represents 20 um).