Transgenic worms carrying
unc-32::GFP plasmid (pHJ-U32P01) were fixed with 4% paraformaldehyde in phosphate-buffered saline. Fluorescence confocal (M, and O) and merged images with phase contrast (N, and P) were acquired. M-P, L1 (M and N) and adult (O and P) worms harboring the
unc-32::GFP plasmid (pHJ-U32P01). In L1 larvae, the
unc-32::GFP gene was highly expressed in the nerve ring (M, arrow) and the ventral nerve cord (M, arrowhead). The signal was clearly observed in the spermathecal-uterine valve, which is located between the uterus and the spermatheca (O, arrowhead). Ph, pharynx; NR,nerve ring; VNC, ventral nerve cord; SUV, spermathecal-uterine valve; Sp, spermatheca. Scale bars, 50 μm.