(F-G') b-tubulin TBB-4::GFP relative ciliary levels and ciliary localization. In WT, TBB-4::GFP localizes to CEM cilia in a stereotypical pattern; lines indicate two offour CEM cilia, quantified in (G), n = 25 cilia. Ciliary TBB-4::GFP levels were normalized to levels in the CEM dendrite, mean fold intensity +- SD. This pattern isaltered in
tba-6 mutants, quantified in (G0), n = 20 cilia. In
tba-6, TBB-4::GFP ciliary levels are significantly reduced and TBB-4::GFP accumulates at ciliary bases.(F0) N = 86 and 102 animals for WT and
tba-6 respectively, p value < 0.0001 by Mann-Whitney U test, Scale bar, 10 um.See also Figure S2 and Table S1.