Fig 4. SUP-17 is localized both to the cell surface and in the cytoplasm in multiple cell types, including known Sma/Mab signalreceiving cells. (A) A schematic of the
sup-17 genomic region (not to scale), depicting the location of various protein domains, the GFP insertion site and the location of the
n1258 and
n316 molecular lesions. (BI) Confocal fluorescent images (B, D, F, H) and corresponding DIC images (C, E, G, I) of transgenic worms expressing endogenous SUP-17::GFP (B, D, F, H) in the early embryo (B), gravid adult gonad (D), L4developing vulva (F) and L3 hypodermis (H). (J) An L3 transgenic animal carrying both over-expressed SUP-17::GFP and
sma-6p::NLS::mRFP, showing expression of SUP-17::GFP in the hypodermal cells (arrows). (K) An L1 transgenic animal carrying both over-expressed SUP-17::GFP and the M lineage specific reporter
hlh-8p::nls::mCherry at the 16-M stage. Some M lineage cells are out of the focal planes shown. (L-M) GFP (L) and DIC (M) images of a mid-stage transgenic embryo carrying over-expressed SUP-17::GFP, showing GFP expression in the intestinal precursor cells (marked by arrows). Anterior is to the left. Scale bars represent 10um in B-M. (N) Rescue of thesmall body size of
sup-17(
n1258) worms by tissue-specific
sup-17 cDNA expression. The mean body length of WT worms is normalized
to1.0. Error bars represent 95% confidence intervals for the normalized body length.