Figure 1. SUN-1 Forms Aggregates at the Nuclear Envelope during the Time of Ongoing Homolog Pairing (A) A mtf-1
) hermaphrodite gonad expressing SUN-1::GFP (middle, green in merge) was stained for DNA with DAPI (top, blue in merge). (B) SUN-1 staining (iii, red in merge) of hermaphrodite gonads expressing ZYG-12ABC::GFP (ii, green in merge). i, DAPI; iv, merge. The yellow arrowhead marks the position of a patch, while the yellow arrow highlights a focus. (C) DAPI staining (i, iii) and antibody staining (ii, iv). PC-binding proteins HIM-8 or ZIM-3 (red) are shown in combination with SUN-1::GFP (green). The green channel is shifted to the right to allow better visualization of green and red signals. (D) Centrosome marked by SPD-5 staining (red) in mitotic (i) and meiotic (ii, transition zone) nuclei expressing SUN-1:: GFP (green). Scale bars represent 10 um. (E) Western blot to show the in vivo interaction of ZYG12ABC::GFPand endogenous SUN-1 (54 kD, red arrowheads). Coimmunoprecipitations were performed with antiGFP antibodies. SUN-1 was detected with anti-SUN-1 antibodies. Genotypes are on the right.