Figure 2
egl-46 rescue of BAG neuronal fate defects. (A) Schematic representation of the
egl46 genomic locus. The ATG codon is marked with an arrow and the exons are represented as black blocks. The
egl-46 translational reporter was constructed by driving
egl-46 genomic DNA with dsRed2 coding sequence under the control of the 4.5-kb
egl-46 promoter. The transcriptional reporter was constructed using the 4.5-kb promoter to drive nuclear-localized dsRed2 (NLS::dsRed2). (B) The 4.5-kb
egl-46 promoter drives NLS::dsRed2 expression in multiple nuclei in the head. Colocalization (yellow) of NLS::dsRed2 was observed with cytoplasmic gfp driven by an
egl-13 promoter in the BAG neurons. Note that we only rarely observed colocalization with the BAG marker, suggesting that the
egl-46 promoter drives expression in the BAG neurons in a transient manner. Ventral view, anterior is to the left. Bar, 20 um. (C) Transgenic expression of the
egl-46 cDNA or
egl-46 genomic sequence fused to dsRed2 under the control of the
egl-46 promoter rescues the
egl-46 (
gk692) mutant loss of
flp19prom::gfp expression. n . 50. ****P , 0.0001. # refers to independent transgenic lines.