Figure 4. Expression pattern of FOZI-1 in wild-type,
cc609 and
cc610 hermaphrodites and embryos. All images are lateral views with anterior to the left and dorsal up (unless otherwise noted). (A-D) Wild-type (A,B) and
cc609 (C,D) embryos stained with anti-FOZI-1 antibody (A,C) and DAPI (B,D). Nuclear FOZI-1 is seen in a subset of cells at the 2-fold stage of embryogenesis in wild-type (A) but not in
cc609 (C) embryos. (E,F) FOZI-1 staining in a wild-type L1 larva on two different focal planes. FOZI-1 was observed in the nuclei of 7-12 head neurons (arrow, E) and 5-7 cells along the ventral nerve cord (arrowheads, E,F) and in cells derived from the M lineage (red box, E,F). (G-R) Double labeling with anti-FOZI-1 antibody and
hlh-8::gfp at the 2-M (G-I), 16-M (J-L), 18-M (M-O) and 2-SM (P-R) stages in wild-type animals. Anti-FOZI-1 antibody staining is shown in panels G,J,M,P;
hlh-8::gfp is shown in panels H, K,N,Q; the corresponding merged images are shown in panels I,L,O,R. FOZI-1 staining was first detected at the 2-M stage (G-I) and persisted through the 16-M (J-L) stages. At the 18-M stage (M-O) FOZI-1 is still present in all undifferentiated CC and BWM precursors but is absent in the SMs. Arrowheads in panel M denote cells in the ventral nerve cord. (P-R) FOZI-1 is not present in the 2-SM cells and the subsequent SM lineage (data not shown). (S) Summary of FOZI-1 expression in the M lineage. The wild-type M lineage is shown with overlay of FOZI-1 expression highlighted in red. (T,U) Staining of
cc609 (T) and
cc610 (U) animals at mid-L1 stage using anti-FOZI-1 antibodies. (T) No FOZI-1 was detected in
cc609 animals. (U) Wild-type level and localization pattern of FOZI-1 are detected in
cc610 mutant animals in head neurons (arrow), ventral nerve cord (arrowheads) and the M lineage (red box).