Figure S5. PLK-1 is dispensable for nuclear envelope patch formation and homolog interactions during meiosis, but is required for mitotic division in the germline. Related to Figure 6. (A) Projection images of
plk-1(RNAi) transition zone nuclei reveal nuclear envelope patches marked by PLK-2 (red) and SUN-1 (yellow). Absence of PLK-1 was verified by immunostaining (not shown). (B) Projection images of
plk-1(RNAi) pachytene nuclei show that PLK-1 is not required for synapsis. SYP-1 (green) localizes along all chromosome axes, marked by HTP-3 immunofluorescence (red). (C) Projection images of DAPI-stained nuclei in the distal, premeiotic region of the germline, indicated by the red box in the diagram below. Premeiotic nuclei in
plk-2 mutants show normal morphology. Depletion of
plk-1 by RNAi results in cell cycle arrest and polyploid nuclei. These mitotic defects are more extreme when
plk-1 is depleted in
plk-2 mutants. (D) Projection image of a wild-type prometaphase nucleus in the mitotic zone stained for PLK-2 (red) and PLK-1 (green). Both proteins are detected at kinetochores and centrosomes. Scale bars, 5um.