Figure 4. WDR-23 expression pattern and site of action. (A) Representative images showing fluorescence in the indicated tissues from transgenic animals expressing nuclear-localized gfp from the endogenous 1.3 kb
wdr-23a promoter fragment. Arrowheads indicate neuronal cell bodies. (B-D) Paralysis on 1.0 mM aldicarb of the indicated strains. For rescue and knockdown, the following promoters were used:
ges-1 (intestine),
rab-3 (neuron),
col-12 (hypodermis), and
myo-3 (muscle). Intestinal and neuronal rescues were independently verified using
nlp-40 and
snb-1 promoters, respectively (data not shown). (B) Rates of paralysis of tissue-specific rescue of
wdr-23 mutants. (C) Hairpin RNAi knockdown of
wdr-23 in a
sid-1(
qt2) background results in tissue-specific aldicarb resistance after 90 minutes on aldicarb (Student's t-test).
wdr-23 indicates knockdown with endogenous
wdr-23a promoter. (D) RNAi knockdown by feeding of the indicated genes in a wild type or neuronally sensitized (
nre-1 lin-15b) strain. Control is the empty expression vector L4440. (Scale bar represents 10 mm; Error bars indicate 6SEM. *p,0.05, **p,0.01.)