Figure 8. MBK-2::GFP and WGA-G colocalize, and
chs-1 is required for MBK-2 localization. (A) Wild type expressing MBK-2::GFP, stained for DNA (Hoechst), GFP (MBK-2) or WGA-binding epitopes, in the germline (top row), or in embryos at meiotic metaphase I (middle row) or meiotic anaphase I (bottom row). In embryos, maternal DNA is shown enlarged in box on lower right hand of DNA images. (B) DNA (Hoechst) or WGA-binding epitopes in
mbk-2(
pk1427) (putative null). (C)
chs-1(RNAi) expressing MBK- 2::GFP and stained for DNA (Hoechst), GFP (MBK-2) or WGA-binding epitopes in the germline (top row), or in an early embryo. (D)
cej-1+B0280.5(coRNAi) expressing MBK- 2::GFP stained for DNA (Hoechst), GFP (MBK-2) or WGA- binding epitopes in the germline (top row), or in an early embryo. (E) DNA (Hoechst) or chitin in germline of wild type. (A-E) Proximal refers to the region of the germline near the spermatheca. Embryos are at the one-cell stage.