Figure 6.
vha-12 is required for embryonic development (A) Normal somatic and germline morphology is observed in
vha-12(
mg41) mosaics in which the rescuing array was lost in the P4 germline progenitor cell. The distal gonad, uterus (ut), spermatheca, sperm (sp), oocytes (numbered relative to spermatheca, -1,-2,-3), and ovulation are normal. Inviable embryos are located in the uterus. Scale bar 50 um. (B) Loss of maternal
vha-12(+) expression results in early embryonic arrest.
vha-12(
mg41) maternal(-) embryos produced from
vha-12(
mg41) germline clones from EG7188
vha-12(
mg41); oxEx1703 hermaphrodites. Multinucleated cells were frequently observed. Animals arrested with less than 40 cells (560 embryos from germline clones). Scale bar, 20 um. (C) and (D) Expression of GFP driven by the
vha-12 promoter is not detected in early embryos (approximately between 0 and 200 minutes after the first cell cleavage). Images from a movie of embryonic development from a Pvha-12::GFP transgenic strain. In each row a transmitted light image of the embryo, at a single plane (C), is shown along with the projected image acquired from the GFP channel (D). Scale bar 20 um. (E)
vha-12(
mg41) homozygotes arrest between two- and three-fold. Three panels from a time- lapse movie taken in a single focal plane of
vha-12(
mg41)
lon-2(
e678) embryos (arrows) produced from a
vha-12(
mg41)
lon-2(
e678); mnDp31 hermaphrodite. See also supplemental movie file S6.