Figure S5, related to Figure 2.
lite-1 expression rescues the defects of
lite-1 mutants, andlite-1 is expressed in 29 cells.(A) The
lite-1 gene, showing the design of several
lite-1 transgenes. The
lite-1 gene model wassemi-automatically constructed using the Exon-Intron Graphic Maker (available at
http://www.wormweb.org).(B) The
lite-1 genomic locus rescued the pumping inhibition defect of
lite-1(
ce314) mutants.(C) The
lite-1prom::
lite-1::gfp transgene rescued the pumping inhibition defect of
lite-1 mutants.(D) The
lite-1prom::gfp::
lite-1 transgene rescued the pumping inhibition defect of
lite-1 mutants.(E) Quantification of acute response latency.(F) Quantification of acute response amplitude.(G) Quantification of recovery response amplitude.(H) Strains carrying all three
lite-1 transgenes were rescued for the light avoidance defect of lite1mutants. Body bends were scored during 10 s of light exposure.(I) Expression pattern of the transcriptional fusion (
lite-1prom::gfp) in the adult head and tail. Noexpression was observed in the midbody.(J) Dorsal view of the transcriptional fusion expression pattern in the adult head.(K) Expression pattern of the C-terminal translational fusion (
lite-1prom::
lite-1::gfp) in the adulthead and tail. No expression was observed in the midbody.For behavioral assays, n = 60 worms. Shading around traces and error bars indicate s.e.m. * p <0.05, ** p < 0.01, *** p < 0.001, ns = not significant, t-test, compared to the wild type or to thestrain indicated. d = dorsal, a = anterior, r = right. Scale bars, 8 um.