Figure S1. Genomic Structure and Developmental Expression Pattern of
inx-16. (A) The
inx-16 locus. The top portion shows the location of
inx-16 on chromosome I. The deficiency (Df) used for mapping and the rescuing cosmid are shown below the chromosome. The extent of the PCR rescuing fragment, containing both
inx-16 and
inx-17, is depicted above the genomic structure of the loci.
inx-17 is downstream of
inx-16 in an operon. The insertion site of green fluorescent protein (GFP) in the INX-16:GFP rescuing fusion construct is denoted. Mutations in the
inx-16 alleles are also shown.
ox144 is a C-to-T transition that results in an early stop after amino acid 104 of 372 predicted residues.
tm1589 deletes 470 base pairs of
inx-16 genomic DNA including most of exons I and II and the intervening intron (National Bioresource Project for the Experimental Animal Nematode C. elegans).(B-D) Expression of GFP driven by the
inx-16 promoter (plasmid pMAP4). Fluorescence is observed in the intestine from early developmental stages. All fluorescent figures have been inverted so that fluorescence appears as black on white. In (B), GFP is observed in the developing in- testine of a comma stage embryo. In (C) is a DIC image of embryo shown in (B). In (D), GFP is observed in the intestine of an L1 stage larva.