Figure 3. Expression pattern of
hst-2::GFP transcriptional reporter. (A)
hst-2::GFP is expressed in the developing pharynx (ph) in a 1.5-fold embryo. (B)
hst-2::GFP expression in L2 larva. GFP is detected in the ph, in neurons in the head (arrows), and in ventral and dorsal nerve cords (arrowheads). (C) In adults,
hst-2::GFP is widely expressed in the pharynx, in neurons of the nerve cords (nc), and in the muscle (m). Fluorescence (D, F, and H) and differential interference contrast (E, G, and I) images show developing vulva and gonad. (D and E) In L3 larva,
hst-2::GFP is strongly expressed in the four descendants of the P6.p vulval precursor cell. Anchor cell (AC), which does not express
hst-2::GFP, induces P5.p, P6.p and P7.p to adopt vulval fate. (F and G)
hst-2::GFP expression persists in vulval cells in L4 larva. (H and I)
hst-2::GFP expression in DTC during gonad development in L3 larva. (A) Anterior is top, dorsal right. (B-I) Anterior left, ventral down. [Scale bars: 10 um(A), 200 um(B), 100 um(C), and 10 um (D-I).]