Figure 1. Testing acute and inherited silencing in
rde-3 mutants :A. Schematic of synthetic piRNAi construct. B. Synthetic piRNAs target sites in
him-5. C. Quantification of males frequency after piRNAi against
him-5 in N2 wild type and
rde-3(
ne3370) animals; N2: N=3,
rde-3: N=3 (
him-5 piRNAi and neg. control); Statistics: Mann-Whitney one-tailed test, N2 vs control (P = 0.05),
rde-3 vs control (P = 0.05), N2 vs
rde-3 (P = 0.05). D. Inherited
him-5 piRNAi silencing in N2 and
rde-3 animals; N = 3 all conditions. Negative control: non-targeting piRNAs. The bar above the graph indicates the presence ("yes"), mixed generation ("mixed"), and absence ("no") of a Pmyo-2::mCherry marked extra-chromosomal array expressing synthetic piRNAs targeting
him-5. Each data point is a biologically independent transgenic line for panel C, and the average of three biologically independent transgenic lines for panel D. Error bars indicate the standard error of the mean (SEM).