Figure 4.
lag-2 and
lin-12 expression in the early embryo. All panels are immunofluorescence micrographs. Bar, approximately 10 µm.(A)
lag-2::lacZ expression at the 55-cell stage. Shown is a
lag-2::lacZ transgenic embryo stained with anti-β-galactosidase antibodies in a left-side view with anterior to the left. β-galactosidase expression is also observed in the four ABala granddaughters (designated by arrowheads), visible at the anterior of the embryo. Weak β-galactosidase expression is observed in the two daughters of MSap (MSapa and MSapp) on the ventral side of the embryo. (B)
lag-2::lacZ expression at the 87-cell stage. Shown is a lag- 2::lacZ transgenic embryo stained with anti-β-galactosidase antibodies in a left-side view with anterior to the left. β-galactosidase expression is observed in the eight ABala great-granddaughters, four of which are visible in the anterior of the embryo in this micrograph. Robust β-galactosidase expression is also observed in the two daughters of MSap (MSapa and MSapp; designated by arrows) on the ventral side of the embryo. (C)
lin-12::lacZ expression at the 55- cell stage. Shown is a
lin-12::lacZ;
smg-1 transgenic embryo stained with anti-β-galactosidase antibodies in a dorsal view with anterior to the left. Robust β-galactosidase expression is observed in 24 cells.