Fig. 4. In situ hybridization showing
fer-1 expression in the testis. (A) Hybridization with the
fer-1 antisense probe.
fer-1 message is only detected in the testis and not in the gut or carcass that includes other tissues. Expression starts near the turn of the gonad and is not seen in spermatogonial cells (sg). (B) Control with a
fer-1 sense probe showing no hybridization to the testis. Bar, 50 um (A and B). (C-D) Higher magnification of the testis loop region with paired Nomarski differential interference contrast optics (C,E) and DAPI fluorescence images (D,F). (C,D)
fer-1 expression begins as the cells mature from mitotic spermatogonial cells to primary spermatocytes (1o). (E,F) The
fer-1 message is not detected past the first meiotic division and is not seen in either the secondary spermatocytes (2o) or spermatids (sd). Bar, 10 um (C-F).