Figure S2. EGL-5 suppresses the expression of
ceh-20 cell-autonomously but represses
lin39 and
mab-5 cell-nonautonomously. (A) mxIs28[
ceh-20::YFP] expression in ALM neurons of the wild type and animals carrying
mec-17p::
egl-5 transgene and PLM neurons of the wild type and
egl-5 mutants. RFP expressed from
mec-17 promoter marks the TRNs. Arrows point to the presence of GFP expression, and dashed ovals indicate the position of the cell body that did not express GFP. (B) PLM posterior neurite labeled by antibody staining against MEC-18 proteins in various Hox mutants. (C) The absence of wgIs18[
lin-39::EGFP] and wgIs27[
mab-5::EGFP] expression in PLM neurons in both wild type and
egl-5 animals. Dashed circles indicate the position of PLM cell body, labeled by
mec-17p::RFP. (D) smFISH using probes against either
lin-39 or
mab-5 shows no signal in
egl-5 PLM neurons.