Figure 1. Expression of the
egl-5-lacZ Fusion(A) A hermaphrodite Ll larva (O-2.5 hr) showing expression of the extrachromosomal
egl-5-lacZ fusion extending from the most posterior body region into the tail. Similar localized expression was observed in over 95% of animals (n > 50), although the number of cells staining was variable. The arrow points to the HSN, which migrates from the tail to the central body region during embryogenesis. The
egl-5-lacZ fusion is strongly and consistently (in over 95% of cases; n > 25) expressed in the posterior body region and the tail of adults, especially in males.(B) Individual cells expressing
egl-5-/lacZ in an Ll animal (O-2.5 hr). mu, body wall muscle (S. J. Salser and C. K., unpublished data). Cells were initially identified using a compound Nomarski microscope. The entire expression pattern of this animal was recorded and reconstructed from photographs by S. J. Salser of a series of focal planes, one of which is shown here. We identified some or all of the staining cells in approximately 20 animals. Fewer cells were observed to stain in most of these animals than in the animal shown here. Cells expressing lacZ usually (in over 90% of cases) included subsets of B, U, Y, K, F, and body muscles in the tail and posterior body region.(C) Schematic diagram of cells affected by
egl-5 mutations in male and hermaphrodite Ll larvae (Chisholm, 1991).