Figure 2.
dnj-11 Encodes a Member of the Family of MIDA1-Like Proteins (A) (Top) Genes and single-nucleotide polymorphisms (SNPs) used for mapping
bc212 are indicated. (Middle) Cosmids assayed for rescue of
dnj-11(
bc212) are shown. (Bottom) Pdnj-11dnj-11 is a subclone of F38A5 and represents the minimal
dnj-11(
bc212) rescue fragment. The J domain, M domain, and Myb domains of the
dnj-11 ORF are indicated in orange, blue, and maroon, respectively. (B) The alignment of the DNJ-11 sequence with the sequence of mouse MIDA1, human MPP11, V. cateri GlsA, and the S. cerevisiae Zuotin was performed using the DIALIGN algorithm. Identical amino acids and similar amino acids are shaded in black and gray, respectively. The orange box marks the J domain and the conserved tripeptide is shaded in orange. The blue box indicates the M domain. The maroon bars above the sequences represent the Myb domains. Arrows point to conserved aromatic residues in the Myb domains.
bc212 results in the truncation of the DNJ-11 protein after aa 6 (asterisk).
tm2859 results in a frame shift after aa 116 (red asterisk) and a stop after an additional 67 aas in a different frame. (C) Embryos transgenic for the functional Pdnj-11dnj-11::gfp transgene were fixed as described in Materials and Methods and stained with an anti-GFP antibody (antiGFP) to detect DNJ-11::GFP and with DAPI (DAPI) to detect nuclei.