Figure 5.
cebp-1 Protein and mRNA Are Localized in Touch Neuron Axon and Synapses(A) Expression of functional GFP::CEBP-1 in touch neurons. GFP is present along the axon (left panel), in the cell body (middle) and synapse (right).(B) GFP::NLS::MS2 reporter detects mRNA of
cebp-1 in touch neuron axons. Illustrations of the constructs are shown above each image. Axonal fluorescence intensity as a fraction of peak cell body intensity is plotted versus distance along the axon from the cell body. GFP::NLS::MS2 alone (juEx1982) is concentrated in the cell body and faintly seen along the axon. Coexpression of juEx1982 with a
cebp-1 transgene containing six MS2-binding sites in the
cebp-1 30 UTR (juEx1984), but not with a control transgene containing six MS2-binding sites in the
unc-54 30 UTR (juEx2013), results in increased punctate GFP in axons. Axonal expression of GFP::NLS::MS2 + CEBP-1-
cebp-1 30 UTR is decreased in
dlk-1(lf) animals but significantly elevated in
rpm-1(lf) animals. (C) Average GFP::NLS::MS2 fluorescence intensity in axons when coexpressed with juEx1984 as % of cell body intensity (n = 15 per genotype). Data shown as mean ± SEM; statistics, Anova. Scale, 10 μm.