Figure 1: The GLR-1 phenotypes of the
daf-7(
e1372) mutant are present at 15C. Experiments were performed with young adults that were maintained at 15C. (A-C) Imaging and quantification of GLR-1::GFP in the ventral nerve cord (VNC) of wild type and
daf-7(
e1372) mutants (n=27 images for each genotype). (A) Representative images of GLR-1::GFP in the VNC of wild type and
daf-7(
e1372) mutants. A subset of the GLR-1::GFP puncta are indicated with white arrows. (B) Quantification of GLR-1::GFP puncta intensity, p=0.008. (C) Quantification of GLR-1::GFP puncta width, p=0.002. (D) Average number of spontaneous reversals per minute for wild type and
daf-7(
e1372) mutants (n=11 for each genotype), p=0.000003. Error bars denote SEM, **p<0.01, ***p<0.001 (Student's t-test).