Figure 1. Loss of CGT does not impair the beneficial effects of B subtilis:A) Survival curves of wild-type (N2) and cgt mutants (
cgt-1(
tm1027),
cgt-1(
ok1045),
cgt-2(
tm1192),
cgt-3(
tm504)) fed on E. coli (OP50; yellow) or B. subtilis (3A1T; blue). Worms were tracked for lifespan starting at L4 stage (day 0) and scored every 2 days. Bagged or missing worms were censored (indicated by a crossline). Significant differences were found between survival of animals treated with E. coli vs B. subtilis. The bottom right panel in (A) shows the survival curves of all animals fed E. coli (OP50) on the same graph. B) Table showing sample size, mean, standard error deviation, & median values for the lifespans of worms grown on the control bacteria (E. coli) and experimental bacteria (B. subtilis). For B, * indicates significant difference compared to the respective animal on B. subtilis and # indicates significant difference compared to N2 on E. coli. C) Acute stress response survival curves of wild-type (N2) and cgt mutants treated with 100mM paraquat. Experiments were performed on 1-day, 5-day, and 10-day old animals. Worms were grown to respective ages on either E.coli (OP50; yellow) or B. subtilis (3A1T; blue) bacterial lawns prior to the stress test. For all, survival curves were analyzed using Kaplan-Meier estimates, and pairwise comparisons were performed using a log-rank test.