Figure 2. SUP-35::GFP expression. (A-D) DIC (A,C) and corresponding GFP (B,D) images of SUP-35::GFP (fdEx57) expression in embryos. The SUP-35::GFP reporter encodes a full-length functional fusion protein that is expressed under the control of the native
sup-35 promoter. Prior to overt morphogenesis (A,B), SUP-35::GFP expression is predominantly cytoplasmic. After the initiation of body morphogenesis, SUP-35::GFP becomes enriched in nuclei (C,D). The developing primordial pharynx is outlined by dashed lines in C and D. The embryos depicted are homozygous for a mutation in
sup-36(
e2217) identical results were obtained using an independently generated array (fdEx58). Identical results were also obtained using analogous arrays in the
sup-37(
e2215)background. Anterior is to the left. Scale bar in A, 10 um for A-D.