Figure 3. The UNC-41 proteins are localized to synaptic regions and colocalize with synaptic vesicle proteins. (A) Wild-type and
unc-41(
e268) animals were stained with antibodies to UNC-41 (green) and UNC-10/RIM (red). UNC-41 is specifically associated with synaptic regions: heavy punctate staining is observed in the nerve ring (nr), and the dorsal (dnc) and ventral (vnc) nerve cords. The UNC-41 proteins are absent in
unc-41(
e268) animals. Head region, anterior is to the left, ventral is down, and the bar is 10 mm. (B) Magnified view of anterior sublateral (SAB) nerve cords stained with antibodies to UNC-41 (green), synaptotagmin (SNT-1; red), and UNC-10/RIM (blue). UNC-41 staining overlaps with SNT-1, but does not colocalize significantly with UNC-10/RIM. Scale bar is 5 um.