Figure 3. MAB-3 Negatively Regulates
ref-1::gfp. (A and B) Conserved regulatory elements containing MAB-3 binding sites are required for rescue of
ref-1.(A) Plot of VISTA alignment of C. elegans and C. briggsae
ref-1 genomic sequences, showing sequence elements (A, B, and C) conserved 50%. Elements A and B contain putative MAB-3 binding sites. Bases matching the MAB-3 binding consensus are blue.(B) Schematic diagrams of
ref-1 PCR fragments and GFP reporter constructs. Positions of
ref-1(lf) mutations and rescuing activities of transgenes are indicated.(C) Wild-type L4 males express
ref-1pro::gfp in spicule-associated cells, but not the seam lineage.(D)
mab-3(
e1240) L4 males express
ref-1pro::gfp in seam and spicule cells.(E) Wild-type L4 hermaphrodites express
ref-1pro::gfp in posterior seam cells.(F) Wild-type L3 males lack expression of ref-1del5::gfp in the ray lineage.(G)
mab-3(
e1240) L3 males express ref-1del5::gfp in abnormal seam lineages.(H) Wild-type L3 hermaphrodites express ref-1del5::gfp in posterior seam cells. Arrowheads denote seam cell nuclei. Scale bars equal 10 μm.