- diplo X gamete
Animals produce ova/sperm that contain 2 X chromosomes.
- temperature induced dauer formation variant
Animals exhibit variations in the entry into the dauer stage when exposed to temperatures that induce dauer formation in control animals. In C. elegans, many Daf-c animals enter dauer at 25C, whereas Hid animals enter dauer at 27C.
- octopamine metabolism variant
Animals exhibit variations in any chemical reaction or pathway involving octopamine, 1-(p-hydroxyphenyl)-2-aminoethanol, compared to control.
- three fold arrest
Cessation of development that normally occurs in C. elegans 520-620min after the first cleavage at 20C, the stage between the 2-fold and fully elongated embryo.
- one point five fold arrest emb
Cessation of development that normally occurs in C. elegans 420-460 min after the first embryonic cleavage at 20C, the stage between the comma and 2-fold embryo.
- EdU incorporation reduced
Cells display reduced incorporation of the thymidine analogue 5-Ethynyl-2'-deoxyuridine (EdU), suggesting reduced cell proliferation. Incorporation of EdU is an indication of active DNA synthesis and thus an indication of cells proceeding through the cell cycle.
- DAPI staining variant
Animals exhibit variations in the pattern of a chemical nuclear stain which is commonly used in fixed specimens and produces a bright blue label, compared to control. DAPI (4'-6-Diamidino-2-phenylindole) forms fluorescent complexes with natural double-stranded DNA (Wormatlas).
- precipice response variant
Animals do not respond to the edge or gap in the medium surface they were supported on in the same manner as controls. In Mitchell et al., the precipice response was defined to be that within 2 seconds of the nose moving off the edge of the agar chunk, the worm begins a reversal that completes at least one full sine wave.
- deirid sensillum morphology variant
Animals exhibit variations in the quantity, structure or organization of the components of the sensilla situated laterally in the anterior (ADE) or the posterior (PDE) of the animal compared to control. In C. elegans, each of the 4 deirid sensilla (2ADE, 2 PDE) contains a pair of neurons with ciliated dendritic processes that lie in a channel formed by a socket cell and invaginated sheath cell. The cilia of the deirids terminate in the subcuticle and are not exposed to the outside; however occasionally take up FITC.