Animals do not respond with sleeping behavior induced through the EGF pathway. The EGF-induced sleep pathway is thought to represent a distinct molecular pathway from developmentally linked sleep. The EGF-induced sleep state occurs in two contexts: by overexpressing the EGF ortholog (LIN-3C), or by EGF signaling after stress (such as temperature elevation) in wildtype animals.
Animals respond with sleeping behavior induced through the EGF pathway, more rigorously than control animals. The EGF-induced sleep pathway is thought to represent a distinct molecular pathway from developmentally linked sleep. The EGF-induced sleep state occurs in two contexts: by overexpressing the EGF ortholog (LIN-3C), or by EGF signaling after stress (such as temperature elevation) in wildtype animals.
Cessation of development that normally occurs in C. elegans 520-620min after the first cleavage at 20C, the stage between the 2-fold and fully elongated embryo.
Cessation of development that normally occurs in C. elegans 420-460 min after the first embryonic cleavage at 20C, the stage between the comma and 2-fold embryo.
Cells display reduced incorporation of the thymidine analogue 5-Ethynyl-2'-deoxyuridine (EdU), suggesting reduced cell proliferation. Incorporation of EdU is an indication of active DNA synthesis and thus an indication of cells proceeding through the cell cycle.
Animals exhibit variations in the pattern of a chemical nuclear stain which is commonly used in fixed specimens and produces a bright blue label, compared to control. DAPI (4'-6-Diamidino-2-phenylindole) forms fluorescent complexes with natural double-stranded DNA (Wormatlas).