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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of streptomycin sulphate in water solutions on the nematode life span. In this experiment streptomycin sulphate was used in following dilutions: 1.0 and 0.1 mg/ml. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without streptomycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21C and in the darkness.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of sodium salicylate in water solutions on the nematode life span. In this experiment sodium salicylate was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without sodium salicylate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without sodium salicylate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with sodium salicylate in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
1998]
C. elegans larvae (1-2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, centrifuged and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for the control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and with or without larvae extract) during 12 hours, then they were transferred in next wells everyday (one worm in one well). Dilutions of larvae extract (1:10, 1:100 and 1:1000) were applied on third day. This investigation was carried out in temperature +24C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
1977]
There are only three out of the adult complement of five classes of motor neurone present in the ventral cords of L1 juveniles. The total of 15 cells is made up of 5 DB's, 6 DA's and 4 DD's. The sequence of cell types is invariant in juvenile cords thus enabling cells to be identified in live animals by their relative positions in the cord. All cells of a particular class were killed by means of a laser microbeam and any subsequent alterations in behavior observed. It was found that larvae with ablated DA's could not back up but moved forward normally, whereas those without DB's had difficulty moving forward but showed normal backing behavior. The latter effect is somewhat masked by head movements which were always normal. Animals without DD's were severely uncoordinated when moving in either direction. These observations are in accord with the predictions of Dick Russel's model of the functioning of the ventral cord.
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[
Worm Breeder's Gazette,
1996]
It is well known that the royal jelly markedly prolongs the life span of honey bees. This substance is recommended by physicians for increasing of life quality. In this experiment royal jelly extract was used in the form of "Apilac" in dilutions 0,02% and 0,002%. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without royal jelly extract) during 6 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Beginning from third day in the whole experiment was used the medium without this royal jelly extract. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Control group Experimental Experimental group (0,02%) group (0,002%) n = 11 n = 11 n = 12 Mean +/- S.D. Mean +/- S.D. Mean +/- S.D Longevity (days): mean 19,4 +/- 1,2 18,0 +/- 1,7 21,8 +/- 1,2 maximal 25 27 26 minimal 10 9 14 Periods (days): prereproductive 2 2 2 reproductive 6,5 +/- 0,5 7,4 +/- 0,6 5,9 +/- 0,4 postreproductive 10,6 +/- 1,2 8,5 +/- 1,6 14,0 +/- 1,3 Fecundity: mean 158,8 +/- 6,6 93,9 +/- 9,9 128,9 +/- 3,4 maximal 197 179 153 minimal 115 60 111
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[
Worm Breeder's Gazette,
1997]
In this investigation nematodes C. elegans lived in liquid me- dium with E. coli and water solution of royal jelly extract in concentration 1:100 000 000 in order to study its effect on longevity of these worms in comparison with control, where ne- matodes lived without this extract. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without royal jelly extract) during 4 hours, then they were discarded and newborn larvae we- re transferred in next wells (with royal jelly extract in any concentration) every day (one worm in one well). This investi- gation was carried out in room temperature and in the dark- ness. The obtained results were as follows (mean and standard error): Control group Experimental group Longevity (in days) Mean 17,19 +/- 1,03 16,31 +/- 1,08 Maximal 29 30 Minimal 8 7 Number of animals 36 35 Conclusion: The water solution of royal jelly extract has no effect on C. elegans longevity.
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[
Worm Breeder's Gazette,
1996]
C. elegans (1 - 2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). The medium with extract from young worms was used in experimental group beginning from 12th day, that is practically in postreproductive period of nematode life span. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.