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[
Worm Breeder's Gazette,
2001]
The purpose of this study was to investigate the effect of different concentrations of sodium fusidine in water solutions on nematode life span. In this experiment sodium fusidine was used in following dilutions: 1:10 3 ,1:10 4 ,1:10 5 ,1:10 6 ,1:10 7 and 1:10 8 . Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without sodium fusidine) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with sodium fusidine in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21 0 C and in the darkness. The obtained results are presented in the following table. Concentration of sodium fusidine n Longevity (days) Mean +/- S.E. Maximal Control 36 9,47 +/- 0,76 21 1:10 3 36 10,03 +/- 0,81 23 1:10 4 36 9,44 +/- 0,88 25 1:10 5 36 8,72 +/- 0,51 18 1:10 6 36 9,86 +/- 0,94 26 1:10 7 36 8,86 +/- 0,73 24 1:10 8 36 8,86 +/- 0,73 15 Conclusion: If sodium fusidine solution was applied to C. elegans, it was not able to increase their mean as well as maximal longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of different concentrations of ascorbic acid in water solutions on nematode life span. In this experiment ascorbic acid was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with ascorbic acid in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21 0 C and in the darkness. The obtained results are presented in the following table. Concentration of ascorbic acid n Longevity (days) Mean +/- S.E. Maximal Control 12 13,7 +/- 1,6 28 1:10 1 12 toxic 1:10 2 12 15,4 +/- 1,3 23 1:10 3 12 17,9 +/- 1,6 22 1:10 4 12 22,0 +/- 0,7 29 1:10 5 12 18,6 +/- 1,3 30 1:10 6 12 19,3 +/- 1,3 30 1:10 7 12 16,3 +/- 1,1 27 Conclusion: If ascorbic acid solution was applied to C. elegans , it was able to increase their mean (by 61,0%, p<0,001) as well as maximal longevity in comparison with control in dilution of 1:10 4 . Acknowledgment: The author wish es to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of different concentrations of streptomycin sulphate in presence of ascorbic acid (concentration 1:10 4 ) in water solutions on the nematode life span in reproductive period. In this experiment streptomycin sulphate was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, from 3th to 10th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21 deg C and in the darkness. The obtained results are presented in the following table. Concentration of streptomycin sulphate n Longevity (days) Mean +/- S.E. Maximal Control 12 12.8 +/- 1.1 18 1:10 3 12 13.7 +/- 0.7 21 1:10 4 12 14.0 +/- 0.6 23 Conclusion: If streptomycin sulphate solution in presence of ascorbic acid was applied to C. elegans , it was not able to increase significantly (P>0.05) their mean longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of sodium salicylate in water solutions on the nematode life span. In this experiment sodium salicylate was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without sodium salicylate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without sodium salicylate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with sodium salicylate in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 10 mg/L, it was able to increase significantly (P>0.05) their mean longevity in comparison with control to 83.96 percent.
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of streptomycin sulphate in water solutions on the nematode life span. In this experiment streptomycin sulphate was used in following dilutions: 1.0 and 0.1 mg/ml. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without streptomycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21C and in the darkness.