As an acidic polysaccharide, the formation of <i>Hyaluronic acid</i> (HA) is typically <i>Sodium Hyaluronate</i> (SH) for knee repair, oral treatment, skincare and as a food additive. Nevertheless, little information is available on the anti-ageing activity of SH as a food additive. Therefore, we treated <i>C. elegans</i> with SH, then inferred the anti-aging activity of SH by examining the lifespan physiological indicators and senescence-associated gene expression. Compared with the control group, SH (800 &#
x3bc;g/mL) prolonged the <i>C. elegans</i>' lifespans in regular, 35 &#
xb0;C and H<sub>2</sub>O<sub>2</sub> environment by 0.27-fold, 0.25-fold and 1.17-fold. Simultaneously, glutathione peroxidase (GSH-Px), antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) were increased by 8.6%, 0.36% and 167%. However, lipofuscin accumulation, reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased by 36%, 47.8-65.7% and 9.5-13.1%. After SH treatment, athletic ability was improved and no impairment of reproductive capacity was seen. In addition, SH inhibited the blocking effect of <i>
age-1</i> and up-regulated gene levels involving <i>
daf-16</i>, <i>
sod-3</i>, <i>
gst-4</i> and <i>
skn-1</i>. In conclusion, SH provides potential applications in anti-ageing and anti-oxidation and regulates physiological function.