Protein kinase C (PKC) is considered to play an important role in the signal transduction of the eukaryotic system and proposed to be a primary target of TPA. As a part of identifying PKC-like genes to understand the structural and functional relationship between
tpa-1 and PKC-like genes, we carried out Southern hybridization analysis using rabbit PKC cDNA(alpha, and gamma forms kindly provided by Dr. Suzuki/Dr. Ohno, Tokyo Metropolitan Institute of Medical Science) as a probe. Five major bands of 6.0, 4.1, 3.2, 3.0, and 1.2kb and several minor bands were detected in HindIII -digested N2 DNA. Also, to clone PKC-like genes we have screened both a cDNA library (a gift from Dr. Kimble/ Dr. Ahringer) and a genomic library with the same rabbit PKC DNA. We have obtained 2 and 7 clones, respectively. We are testing homology between these cloned DNA's and putative
tpa-1 cDNA as well as a rather long stretch of genomic DNA covering the 2. 4kb fragment associated with Tc1-induced TPA resistance.