We have identified a dominant X-chromosome nondisjunction mutant that differs from those studied previously. It has been shown by pseudolinkage results to be a translocation involving X and IV, which we call mnT12(IV;X). The X breakpoint maps to the right of the right- most marker known,
let-6, and the IV breakpoint appears to be near the left end of IV, i.e., the translocation appears to be a fusion of the right end of X with the left end of IV. Donna Albertson, at the MRC Laboratory of Molecular Biology, has looked at embryonic cells of mnT12 homozygotes and confirmed the existence of a fusion chromosome: she has taken nice pictures of cells showing 10 chromosomes in place of the normal 12, with one pair about twice as large as the normal pairs, which are roughly all the same size. Assuming tips of X and IV have been lost, the losses must not have been extensive because mnT12 homozygotes are viable and fertile. Hermaphrodites heterozygous for mnT12 segregate about 27% males among their self progeny. We have looked for nondisjunction of mnT12 and the normal IV in heterozygous males and have not detected it. The heterozygous males also sire close to equal numbers of male and hermaphrodite progeny. The mnT12 homozygotes are non-Him, as expected, and appear to have little nondisjunction of the mnT12 chromosomes. We are studying recombination between the two fusion chromosomes in the homozygote, and we are inquiring about the stability of the fusion. Finally, it is worth noting that a line of C. elegans can be maintained in which males have a neo-X (fusion chromosome) neo-Y (normal IV) genotype and hermaphrodites have two neo-X chromosomes and no neo-Y, i.e., the organism is converted from an XO type of sex determination to an XY system.