The application of CRISPR technology has greatly facilitated the creation of transgenic <i>Caenorhabditis elegans</i> lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here I describe a new approach for introducing large DNA constructs into the <i>C. elegans</i> genome at specific sites using a combination of Flp and Cre recombinases. The system utilizes specialized integrated landing sites that express GFP ubiquitously flanked by single <i>loxP</i>, <i>FRT</i> and <i>FRT3</i> sites. DNA sequences of interest are inserted into an integration vector that contains a <i>
sqt-1</i> self-excising cassette and <i>FRT</i> and <i>FRT3</i> sites. Plasmid DNA is injected into the germline of landing site animals. Transgenic animals are identified as Rol progeny, and the <i>
sqt-1</i> marker is subsequently excised with heat shock Cre expression. Integration events were obtained at a rate of approximately 1 integration per 3 injected F0 animals; a rate substantially higher than any current approach. To demonstrate the robustness of the approach, I compared the efficiency of the Gal4/<i>UAS</i>, QF(and QF2)/<i>QUAS</i>, tetR(and rtetR<i>)/tetO</i>, and LexA/<i>lexO</i> bipartite expression systems by assessing expression levels in combinations of driver and reporter GFP constructs and a direct promoter GFP fusion each integrated at multiple sites in the genome. My data demonstrates that all 4 bipartite systems are functional in <i>C. elegans</i> Although the new integration system has several limitations, it greatly reduces the effort required to create single copy insertions at defined sites in the <i>C. elegans</i> genome.