New deficiencies on LGIII Heinke Schnabel and Ralf Schnabel, Max-Planck-lnstitut fur Biochemie, D-82152 Martinsried, Fed. Rep. of Germany In an attempt to generate new deficiencies on LGIII (which we need to analyze the maternal-effect lethals that we have generated) we mutagenized
unc-32(
e189)/qC1 worms with 7500R. A total of 5186 F1 animals were picked to single plates and their progeny analyzed for the absence of Unc worms, indicating a lethal mutation linked to
unc-32. 904 had to be discarded for various reasons, 923 were sterile or produced only dead eggs. Of the remaining 3359, 21 stable lethals linked to
unc-32 were obtained after outcrossing. These lethals were then testcrossed with several markers in order to find putative deficiencies. In this manner, we obtained 4 deficiencies which in addition to being lethal fail to complement mutations in the following genes: deletes tDf5 tDf6 and tDf8 tDf7 dpy- 18,
spe-6,
bli-5,
unc-64 unc-25,
unc-71,
unc-64, b li- 5 dpy- 18,
spe-6 complements
vab-7,
tra-1 dpy-18,
spe-6 bli-5,
unc-25, vab- 7 There are three other lethals that each fail to complement mutations in a single gene:
dpy-1,
dpy-18, or
bli-5. These may well be double mutations rather than deficiencies. All of the above will be available from the CGC soon. We thank Michaela Berr for excellent technical assistance.