Use of an expression library to clone genes by complemcntation. RE. Palmer and P.W. Sternberg, HHMI and Division of Biology, Caltech, Pasadena, CA 91125
DNA fingerprinting in C. elegans - an approach Mark Beneckea, Jorg T. Epplenb and Einhard Schierenberga a Zoologisches Institut der Univeritat, 50923 Koln, Germany b Ruhr-Universitat, Ab1. fur Molekulare Humangenetik, 44780 Bochum, Germany
A 4 liter glass 'fermenter' has been used instead of Roux bottles in our lab for C. elegans mass liquid cultures. It is made of glass plates stuck together with silicone rubber. It is autoclavable at 120 C, and provided with an aluminium cover containing two noles, into which tubes, are sealed by silicone rubber. One tube (the shorter one) includes a 'weighted' valve, supplied with a movable but hole- covering glass ball. The other an inverted T, the horizontal arms of which are perforated with holes, the prefiltered condensed air entering via the vertical section. This apparatus is practical for both bacteria and C. elegans. New cultures are started by transferring the cover to a new, sterilized culture from an old one. [See Figure 1]