The
let-7 microRNA (miRNA) is an ultraconserved regulator of stem cell differentiation and developmental timing and a candidate tumor suppressor. Here we show that LIN-28 and the poly(U) polymerase PUP-2 regulate
let-7 processing in Caenorhabditis elegans. We demonstrate that
lin-28 is necessary and sufficient to block
let-7 activity in vivo; LIN-28 directly binds
let-7 pre-miRNA to prevent Dicer processing. Moreover, we have identified a poly(U) polymerase, PUP-2, which regulates the stability of LIN-28-blockaded
let-7 pre-miRNA and contributes to LIN-28-dependent regulation of
let-7 during development. We show that PUP-2 and LIN-28 interact directly, and that LIN-28 stimulates uridylation of
let-7 pre-miRNA by PUP-2 in vitro. Our results demonstrate that LIN-28 and
let-7 form an ancient regulatory switch, conserved from nematodes to humans, and provide insight into the mechanism of LIN-28 action in vivo. Uridylation by a PUP-2 ortholog might regulate
let-7 and additional miRNAs in other species. Given the roles of Lin28 and
let-7 in stem cell and cancer biology, we propose that such poly(U) polymerases are potential therapeutic targets.