[
2011]
The authors note that in the Materials and Methods under the section Actin Pelleting Assay, the 10 stock concentration was listed instead of the 1 final working concentration for the polymerization and reaction buffers. The correct concentrations are as follows: polymerization buffer (20 mM Imidazole pH 7.0, 100 mM KCl, 2 mM MgCl2, 0.5 mM ATP, 1 mM EGTA) and reaction buffer (20 mM Imidazole pH 7.0, 150 mM NaCl, 2 mM MgCl2, 0.5 mM ATP, 1 mM EGTA, 1 mM DTT).
[
Nat Methods,
2019]
The originally published paper has been updated to include the following new reference, added as ref. 18: Albrecht, T., Zhao, Y., Nguyen, T. H., Campbell, R. E. & Johnson, J. D. Fluorescent biosensors illuminate calcium levels within defined beta-cell endosome subpopulations. Cell Calcium 57, 263-274 (2015). Subsequent references have been renumbered in the reference list and throughout the text. Minor text changes were made in the sentence in which this new reference is first cited: "Previous attempts used endocytic tracers bearing either pH- or Ca<sup>2+</sup>-sensitive dyes to serially measure population-averaged pH and apparent Ca<sup>2+</sup> in different batches of cells, thus scrambling information from individual endosomes<sup>13-17</sup>" in the original introduction was changed to "Previous attempts used endocytic tracers bearing either pH- or Ca<sup>2+</sup>-sensitive dyes<sup>13-17</sup> or fluorescent-protein-based sensors<sup>18</sup> to serially measure population-averaged pH and apparent Ca<sup>2+</sup> in different batches of cells, thus scrambling information from individual endosomes." These changes have been made in the HTML and PDF versions of the article.