The Caenorhabditis elegans hermaphrodite is essentially a female that produces sperm. In C. elegans, tra-2
promotes female fates and must be repressed to achieve hermaphrodite spermatogenesis. In an effort to learn how mating systems evolve, we have cloned tra-2
from C. remanei, the closest gonochoristic relative of C. elegans. We found its structure to be similar to that of Ce-tra-2
but its sequence to be divergent. RNA interference demonstrates that Cr-tra-2
promotes female fates. Two sites of tra-2
regulation are required for the onset of hermaphrodite spermatogenesis in C. elegans. One, the MX region of TRA-2, is as well conserved in C. remanei as it is in C. briggsae (another male/hermaphrodite species), suggesting that this control is not unique to hermaphrodites. Another, the DRE/TGE element of the tra-2
3'' UTR, was not detected by sequence analysis. However, gel-shift assays demonstrate that a factor in C. remanei can bind specifically to the Cr-tra-2
3'' UTR, suggesting that this translational control is also conserved. We propose that both controls are general and do not constitute a novel "switch" that enables sexual mosaicism in hermaphrodites. However, subtle quantitative or qualitative differences in their employment may underlie differences in mating system seen in Caenorhabditis.
The authors, Coleman-Hulbert, AL; Johnson, E; Sedore, CA; Banse, SA; Guo, M2; Driscoll, M3; Lithgow, GJ; and Phillips, PC, submit the following correction for 10.17912/micropub.biology.000131
The original text as read &#8220;We assayed lifespan in response to imatinib mesylate exposure in threeCaenorhabditisspecies in triplicate using our previously published workflow (Lucanicet al. 2017a; b). is correct.
The reference Lucanic 2017b et al. is:
Lucanic M, Driscoll M, Plummer WT, Harke J, Chen E, Bhaumik D, Harinath G, Coleman-Hulbert A, Dumas K, Onken B, Johnson E, Fougler A, Guo S, Crist A, Presley M, Xue J, Sedore C, Chamoli M, Change M, Chen M, Angeli S, Royal MA, Willis J, Edgar D, Shobna P, Chao E, Kamat S, Hope J, Ibanez-Ventoso C, Kish J, Guo M, Phillips P, Lithgow G. Standardized protocols from theCaenorhabditisIntervention Testing Program 2013-2016: Conditions and assays used for quantifying the development, fertility and lifespan of hermaphroditicCaenorhabditisstrains. Protoc. Exch. 2017. doi: 10.1038/protex.2016.086.
The following reference is incorrect:
Plummer WT, Harke J, Lucanic M, Chen E, Foulger AC, Onken B, Coleman-Hulbert AL, Dumas KJ, Guo S, Johnson E, Bhaumik D, Xue J, Crist AB, Presley MP, Harinath G, Sedore CA, Chamoli M, Kamat S, Chen MK, Angeli S, Chang C, Willis JH, Edgar D, Royal MA, Chao EA, Shobna P, Garrett T, Ibanez-Ventoso C, Hope J, Kish JA, Guo M, Lithgow GJ, , Phillips PC. Standardized protocols from the Caenorhabditis Intervention Testing Program 2013-2016: Conditions and assays used for quantifying the development, fertility and lifespan of hermaphroditic Caenorhabditis strains. Protoc. Exch. 2017b. 10.1038/protex.2016.086