With the increased aging of the population, the extension of lifespan and the improvement of healthspan have become important. Our previous studies showed that the rice bran peptide KF-8 exerts an antioxidant effect in cells and mice. In this study, we evaluated the effects of KF-8 on the healthspan and lifespan of <i>Caenorhabditis elegans</i>. We found that KF-8 prolonged the life of nematodes and showed no reproductive toxicity towards nematodes. In addition, KF-8 improved the motility of nematodes and resulted in an extended body length. Using hydrogen peroxide and juglone as stress inducers, we found that KF-8 improved the anti-stress ability of nematodes. In addition, KF-8 upregulated the expressions of
skn-1,
daf-16 and antioxidant genes. In addition, the life-prolonging effect of KF-8 was lost in
skn-1 mutant strains and
daf-16 mutant strains, indicating that KF-8 may exert anti-aging effects through
skn-1 and
daf-16.