We have identified a new
her-1 gain-of-function mutation,
her-1(
y101sd).
y101 was isolated as a dominant Egl that segregated abnormal males.
her-1(
y101sd) seems to be a stronger gain-of-function mutation than the previously described
her-1(
n695sd) (Trent et al. Genetics 120:145-157), since at 20 C approximately 60% of
y101 XX homozygotes develop as pseudomales or intersexes compared to approximately 20% for
n695. We have used two criteria to assign the
y101 mutation to the
her-1 gene. First,
y101 is tightly linked (<1 m. u.) to
unc-42(
e270)V, which maps 0.2 map units from
her-1. Second, we have obtained three new recessive
her-1(lf) alleles as revertants of the dominant Egl and Tra phenotypes of
y101. (4000 EMS-mutagenized haploid genomes were examined in this revertant screen, which also yielded four extragenic suppressor mutations as well as two Fem mutations.)