In a recent paper we reported work on the genetic analysis of life span in Clock mutants and numerous double mutants ( Lakowski and Hekimi (1996). Science 272, 1010-1013 ). This work has generated many strain requests, so we have sent the following strains to the CGC: CB4876
clk-1(
e2519) III MQ130
clk-1(
qm30) III MQ125
clk-2(
qm37) III MQ131
clk-3(
qm38) II MQ124
clk-3(
qm38) II;
clk-1(
e2519) III MQ225
clk-3(
qm38) II;
clk-2(
qm37) III MQ141
clk-1(
e2519)
clk-2(
qm37)/dpy-17
(e164) III MQ223
clk-3(
qm38) II;
gro-1(
e2400)/dpy-17
(e164) III MQ224
clk-3(
qm38) II;
clk-1(
qm30)/dpy-17
(e164) III MQ524
gro-1(
e2400)
clk-2(
qm37)/
dpy-17(
e164) III MQ513
daf-2(
e1370)
clk-1(
e2519) III MQ415
age-1(
hx546)
fer-15(
b26) II;
gro-1(
e2400) III Notes on using these strains 1) All Clock mutations are maternally and zygotically rescued even in the double mutant strains. 2) Strains homozygous for
clk-2(
qm37) and
gro-1(
e2400) are not viable at 25C. They can be studied at 25C if they are allowed to complete embryogenesis at 20C or below and then shifted to 25C. MQ130
clk-1 (
qm30) also has difficulty at 25C, segregating many dead eggs, but can be maintained at this temperature. 3) MQ124
clk-3(
qm38) II;
clk-1(
e2519) III is a strain which is just barely viable and can not be maintained at 25C. This strain produces a lot of dead eggs and sporadically becomes sterile during culture. 4) The strains MQ141, 223, 224 and 524 have LGIII Clock mutations balanced over
dpy-17(
e164) because these Clock double mutant combinations are not viable as homozygotes, dying out over a few generations.
dpy-17 was chosen because it lies about 0.2 cM to the left of
clk-1 and
gro-1 and only about 1 cM to the left of
clk-2.
dpy-17 is also convenient because it has a strong phenotype as an L1 larva. Homozygous double mutants should be identifiable because their progeny take so long to hatch and to develop.