We investigated the role of the low-density lipoprotein receptor
lrp-2 in C. elegans vulval cell lineage polarity. We find that despite the high conservation of the Wnt signaling component, LRP5/6, in higher order organisms it appears to have evolved after the split of Nematoda due to its absence in all nematode genomes examined. C. elegans contains multiple low-density lipoprotein receptors within its genome, two of which are
lrp-1 and
lrp-2. Due to the position in the genome and high sequence similarity we believe that
lrp-2 is the product of a recent duplication of
lrp-1 within the Caenorhabditis lineage (Minor and Sternberg, 2019d).
To understand the potential role of
lrp-1 and
lrp-2, we sought to score the vulval phenotypes of these mutants during L4 stage.
lrp-1(
ku156) mutants are sick and arrest during an early larval stage. For this reason we were not able to examine the role of
lrp-1 in vulval formation. Despite high sequence similarity and proposed functional redundancy with
lrp-1,
lrp-2 mutants are remarkably wild-type.
lrp-2 is expressed in the developing vulva at the same time as both
cam-1 and
vang-1. By examining double and triple mutant strains we find that
lrp-2 is likely to function downstream of
egl-20 along with transmembrane proteins
cam-1 and
vang-1 (Minor and Sternberg, 2019b; Minor and Sternberg, 2019c). All three genes antagonize the
lin-17/Frizzled pathway by directing the aberrant localization of SYS-1 to the posterior daughter cell of P7.p, thereby leading to the posterior orientation of the P7.p lineage (Minor and Sternberg, 2019a). These observations suggest the simple model shown in Figure 1, in which LRP-2 cooperates with CAM-1 and VANG-1 to respond to EGL-20.
This work provides evidence that despite lacking a true LRP5/6 ortholog, the formation of the C. elegans vulva is controlled by another member of the low-density lipoprotein superfamily,
lrp-2. This data could potentially lead to insight into the evolution of both structure and function of the highly important Wnt pathway component, LRP5/6. Despite strong genetic evidence, this work does not describe the physical interaction between LRP-2 and CAM-1, VANG-1, EGL-20. Can LRP-2 bind with the other transmembrane proteins, CAM-1 and VANG-1, involved in this pathway? Can LRP-2 physically bind the Wnt ligand, EGL-20? Future work should focus on the biochemistry of this pathway. Answers to these questions could provide interesting insights into the evolution of low-density lipoprotein receptors, including LRP5/6, as well as how Wnt signaling has evolved within nematodes without the presence of one of the most important and highly conserved transmembrane proteins.