The SAX-3::GFP is expressed transiently in most neurons and in some epidermal and muscle cells. In
rpm-1 loss-of-function mutants, the SAX-3::GFP signal is more widely detected. In
rpm-1(+) L4 larvae, SAX-3::GFP is detected in 25% (n=60) of the PLM neurons, whereas in 57% (n=60) of
rpm-1(
ur299) L4 larvae SAX-3::GFP signal is observed. In 23% (n 60) of
rpm-1 loss-of-function mutants, there are abnormal multiple processes from the PLM cell body. Whereas in ventral cord motor neurons the SAX-3::GFP signal is observed in 19% (n=60) of
rpm-1(+) larvae, 76% (n=60) of
rpm-1 loss-of-function mutants show strong expression. In the
rpm-1(+) background, SAX::GFP is observed in the ALM and PLM neurons as a punctate pattern in 88 and 84%, respectively, of the animals (n=103). However in
rpm-1(+) mutants (n=105), the pattern appears more uniform and the punctate pattern was observed in only 39 and 37% of the ALM and PLM neurons.